QiTissue
Videos
Software Training Compilation
Software Training Compilation
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QiTissue
↳ Welcome to the QiTissue Help Pages
↳ Application Performance and QiCloud Integration
↳ User Interface
Download
What's New?
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Hardware Requirement Specifications
↳ Operating System
↳ CPU
↳ GPU
↳ RAM
↳ Storage
↳ Display
↳ External Display
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Videos
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Software Training Compilation
↳ Tutorial
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Project Browser
↳ Basic Project Browser Tutorial
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Basic Image Display
↳ Basic Image Display Tutorial
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Sequencer
↳ Basic Sequencer Tutorial
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Feature Table
↳ Basic Feature Table Tutorial
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Correlation Matrix
↳ Correlation Matrix Tutorial
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Workflow Editor
↳ Basic Workflow Editor Tutorial
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UMAP, t-SNE, and Clusters
↳ Basic UMAP, t-SNE, and Clusters Tutorial
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Scatter Plot and Gating
↳ Basic Gating Tutorial
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Segmentation
↳ Basic Segmentation Tutorial
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Feature Plot
↳ Basic Feature Plot Tutorial
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Getting Started
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Naming your Datasets
↳ What is Cyclic Immunofluorescence?
↳ File Naming Structure
↳ Create Your Custom Naming Scheme
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Using the Project Browser
↳ New and Cached Datasets
↳ What is Cache?
↳ Memory Management
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Key Folder Locations
↳ Application Support
↳ User Data Directory
↳ Image Cache Directory
↳ Shortcut to access hidden folders
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Image Display & Visualization Control: The Basics
↳ Image Contrast
↳ Adjust Image Curve
↳ Thresholding
↳ Export Display Settings
↳ Channel List
↳ Favorites
↳ Registration
↳ 3D View
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Workflow Layouts
↳ Processing Layout
↳ Visualization Layout
↳ Analysis Layout
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Feature Data
↳ Why Annotate?
↳ Segmentation
↳ Analyzing Feature Data
↳ Gating
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Documentation
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License Management
↳ Moving Your License to a New Computer
↳ Non-Networked Computers
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Project Browser
↳ Organizing Image Data
↳ Navigating Your Files
↳ View Scenes in a Grid
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Channel Adjustment
↳ Channel Right-Click Options
↳ Color Palette
↳ Channels List Icon Bar
↳ Color Scheme Presets
↳ Channel Grouping
↳ Contrast Options
↳ Quality Analysis
↳ Channel Favorites
↳ Channel Table
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Histogram
↳ Image Display Settings
↳ Adjusting the Curve
↳ Hightlight a Data Range
↳ Blink Effects
↳ Default Blink Effect
↳ Slow Fade Blink Effect
↳ Y-Axes
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View Navigator
↳ Adjusting Field of View
↳ 2D Navigation
↳ 3D Mode and Height-Maps
↳ 3D Navigation
↳ Wobble Effects
↳ Horizontal, Vertical, and Circular Wobbles
↳ Multiple Image Views
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Registration
↳ Automatic Registration
↳ Manual Registration
↳ Registration Settings
↳ Check Registration Settings
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Segmentation
↳ Parameters for Nuclear Segmentation
↳ Fine-Tuning Your Parameters
↳ Segmentation Methods
↳ Measuring Cell and Image Features
↳ Segmenting Regions of Interest
↳ Importing Segmentation Masks
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Properties Panel
↳ Channel
↳ View
↳ Annotation
↳ Cellset
↳ Attributes
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Tools
↳ Drawing
↳ Gating
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Cell Selection Overlays
↳ Importing Cluster Files
↳ Cluster Import File Format
↳ Additional Syntax
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Feature Table
↳ Set a Data Source
↳ Find Cells In Your Image View
↳ Import/Export Feature Data
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Feature Plot
↳ Set a Data Source
↳ Plot Types
↳ Export a Plot
↳ Plot Favorites
↳ Apply t-SNE/UMAP and K-Means Clustering
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Extra Plots
↳ Graphing Signal Levels
↳ Graphing Feature Values
↳ Additional Graphing Options
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Sequencer
↳ Create a Scene
↳ Using the Timeline
↳ Playback Options
↳ Transitions
↳ Examples of Transitions
↳ Sequencer Favorites
↳ Export
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Workflow Editor
↳ Gating Nodes
↳ Merge Nodes
↳ Cluster Nodes
↳ Import Gating Positions and Cell Phenotype Definitions
↳ Future Reuseable Components
Correlation Matrix
Reference Browsers
Diagrams
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Advanced Features
↳ Advanced Registration using ASHLAR
↳ Controls-based Normalization
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Machine Learning Segmentation
↳ ML Segmentation - Cellpose and Stardist
↳ ML Segmentation - Deepcell (experimental)
FAQ
Jump to chapter...
Core functionality
General overview of UI
Loading your dataset in the Project Browser
Adjust contrast settings using the histogram
View channels by rounds/cycles
Apply contrast setting adjustments to the entire list of channels
Registering your image channels
Color channel perspectives and 3D visualization
The favorites system
Running cell segmentation
Plot feature data/gate and apply clustering algorithms
Visualize a gating strategy using the node editor
The biomarker correlation matrix
Managing your memory and disk space
General preferences and cache management